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May Grunwald eosine Methylene blue solution modified 500ml

MERCK
May Grunwald eosine Methylene blue solution modified 500ml

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MOD: 25031-00

May Grunwald eosine Methylene blue solution modified

In Stock
  • Price: 51.50€
  • 41.53€ + TAX 24%

Brand:MERCK

Description

May Grunwald eosine Methylene blue solution modified

Solution
 
1. Buffer solution pH 6.8 (7.2)
Dissolve 1 tablet in 1l distilled water.
 
2. Dilute Giemsa's solution (1:20): Add 10 ml Giemsa's solution to 190ml buffer solution (pH 6.8 or 7.2),  mix well, allow to stand 10 minutes and filter if necessary.
 
For staining automat
 
Slowly add 25ml Giemsa solution to  275ml buffer (pH 6.8 or 7.2) mix  well, allow to stand for 10 minutes and  filter if necessary.
 
Procedure Staining rack Methanol                     3min
 
 
Dilute Giemsa's solution                            15–20min
 
Rinse with buffer solution Drying Coplin jar
 
Blood smears                                                  Air dried
 
Methanol                                                             3–5min
 
Dilute Giemsa solution                               15–30min
 
Wash in buffer solution                                  2 x 1min
 
Drying Staining automat Methanol                    3min
 
Dilute Giemsa solution                              15–30min
 
Wash in buffer solution                                       1min
 
Tap water                                                               2min
 
Drying                                                                     3min
 
Result
 
Nuclei: purple to violet
 
Lymphocytes: plasma light blue
 
 Monocytes: plasma dove blue
 
Granulocytes:
 
- neutrophilic:                                   granules delicate    violet
 
- eosinophilic:                                  granules red to    grey-blue
 
- basophilic:                                     granules strong    violet
 
Thrombocytes:                                                 violet
 
Erythrocytes:                                                  weak red
 
Blood parasites:                                            nulei bright red
 
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