KARYOPREP 65000 Biopsy Vials 10ml

 Advantages of the KaryoPrep Method:

• Cost-Effective Materials: The most economical method on the market.
• Cell Autonomy Maintained: Cells retain their integrity and functionality.
• Fast and Customizable: A quick method that is fully customizable at the protocol stages.
• Single Solution Usage: Only the Karyo-Prep Sample/solution for fixation 10ml vial is required.
• Long Reagent Shelf Life: Reagents have a long shelf life of 5 years from the production date.
• No Need for Additional Automated Equipment: Vortex and centrifuge can be used without the need for further automated equipment.

The method is successfully used in cytology clinics and covers a wide range of sample types:

• Cervical
• Ascitic
• Pleural
• Urine
• Breast
• For molecular processing

Manual Liquid Phase Protocol for Non-Bloody Samples:

1. Vortex the Karyo-Prep Sample for 20 seconds.
2. Transfer the contents of the Karyo-Prep Sample (2ml of the homogenized solution) into a conical urine tube.
3. Centrifuge the conical urine tube at 800G for 10 minutes.
4. Discard the supernatant.
5. Collect the pellet with a Pasteur pipette.
6. Prepare a slide by depositing the pellet onto two glass slides (deposit the sample on one slide, apply the second slide inverted on top, remove it to spread the cytological material into a monolayer on both slides).
7. Spray with Fixative from a distance to avoid displacing the pellet from the slide due to the spray mist.
8. Air-dry the slide for 10-15 minutes or use a hairdryer.
9. Immerse the slide in a coplin jar with 95% histanol for 20 minutes.
10. Perform Pap staining according to the step protocol.
11. Cover the slide with a coverslip.
12. Examine under a microscope at 10x, 40x.

For Bloody Samples:

Follow the above protocol after first using 2ml of Red Blood Lysis Reagent in the Karyo-Prep Sample/solution for fixation 10ml with the sample.