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May Grunwald eosine Methylene blue solution modified 500ml

MERCK
May Grunwald eosine Methylene blue solution modified 500ml

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MOD: 25031-00

May Grunwald eosine Methylene blue solution modified

In Stock
  • Price: 51.50€
  • 41.53€ + TAX 24%
Brand:MERCK
    Description

    May Grunwald eosine Methylene blue solution modified

    Solution
     
    1. Buffer solution pH 6.8 (7.2)
    Dissolve 1 tablet in 1l distilled water.
     
    2. Dilute Giemsa's solution (1:20): Add 10 ml Giemsa's solution to 190ml buffer solution (pH 6.8 or 7.2),  mix well, allow to stand 10 minutes and filter if necessary.
     
    For staining automat
     
    Slowly add 25ml Giemsa solution to  275ml buffer (pH 6.8 or 7.2) mix  well, allow to stand for 10 minutes and  filter if necessary.
     
    Procedure Staining rack Methanol                     3min
     
     
    Dilute Giemsa's solution                            15–20min
     
    Rinse with buffer solution Drying Coplin jar
     
    Blood smears                                                  Air dried
     
    Methanol                                                             3–5min
     
    Dilute Giemsa solution                               15–30min
     
    Wash in buffer solution                                  2 x 1min
     
    Drying Staining automat Methanol                    3min
     
    Dilute Giemsa solution                              15–30min
     
    Wash in buffer solution                                       1min
     
    Tap water                                                               2min
     
    Drying                                                                     3min
     
    Result
     
    Nuclei: purple to violet
     
    Lymphocytes: plasma light blue
     
     Monocytes: plasma dove blue
     
    Granulocytes:
     
    - neutrophilic:                                   granules delicate    violet
     
    - eosinophilic:                                  granules red to    grey-blue
     
    - basophilic:                                     granules strong    violet
     
    Thrombocytes:                                                 violet
     
    Erythrocytes:                                                  weak red
     
    Blood parasites:                                            nulei bright red
     
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